(D, E: n = 30 males per treatment, assay was repeated at least 10 times for each genotype, one-way ANOVA with Tukey’s post hoc test for multiple comparisons). The (D) elav-Gal4 driver was used to express Ets96B RNAi ( Ets96B RNAi1 or Ets96B RNAi2 ) throughout development (UAS- Ets96B RNAi1 and UAS- Ets96B RNAi2) and (E) the elav-GAL4, tub-GAL80 ts driver was used to knockdown Ets96B ( Ets96B RNAi1 or Ets96B RNAi2 ) in adult males. Flies fed ad lib were set as 100%, represented by 1 on the graphs (B, C: n = 10 replicates, one-way ANOVA with Tukey’s post hoc test for multiple comparisons) (D, E) Triglyceride levels were determined in male flies at 0, 12 and 24 hours of starvation. (C) To examine how nutritional state affects the expression levels of Ets96B, RNA was extracted under different nutritional states. (n = 30–60 flies per genotype, one-way ANOVA with Tukey’s post hoc test for multiple comparisons) (B) To examine how starvation affects the expression level of Ets96B, RNA was extracted from normal fed flies, as well as after 24 and 48 h of starvation. 5–7 day old control and Ets96B knockdown males (GAL4 driver crossed to either Ets96B RNAi1 or Ets96B RNAi2) were placed in a vial containing 1% agarose and maintained at 25☌. (A) We tested the effect of Ets96B-knockdown in flies using the starvation survival assay. ![]() Drosophila homologues that were up-regulated when Ets96B was knocked down are in red, and those that were up-regulated more than 2.5-fold are underlined. These include molecular chaperones that function to prevent aggregation (Bip, HSP90B1, DNAJC3, HYOU1 and CALR), protein disulfide isomerases that catalyze disulfide bond formation, isomerization and reduction (PDIA3, PDIA3 and P4HB), and proteins involved in de- or re-glycosylation of improperly folded glycoproteins (PRKCSH and UGGT1). (E) Diagram depicting molecules involved in endoplasmic reticulum protein folding. Genes in red were recovered in the Ets96B SOLiD sequencing. (D) Data showing human homologues of Drosophila genes recovered in Biogrid and String analysis. (C) Biogrid and String were used to find all genes that interact with the molecular chaperone genes recovered in the Ets96B knockdown males. (n = 25 males per treatment *** P<0.005 compared with elav-GAL4 controls, one-way ANOVA with Tukey’s post hoc test for multiple comparisons). This assay was repeated at least 7 times. (B) Relative expression levels of CaBP1, ERp60 and Crc in 5–7 day old control males or males where Ets96B was knocked down in the entire nervous system throughout development. Only genes regulated more than ±log2 2.5-fold change are included. Workflows and tools on commercial Workbenches can only be viewed using a commercial Workbench with a valid license installed.ĬLC Workbenches, including CLC Sequence Viewer, are available for Windows, Mac and Linux platforms.Ets96B RNAi versus controls ( w, elav-GAL4> w 1118 and w 1118> Ets96B RNAi) 5–7 day old male heads. ![]() Viewing Mode does not require a license and can be used for free. If you have access to a CLC Genomics Server, you can also connect to it and view data held on the server. Using the Viewing Mode of CLC Genomics Workbench, you can also view all data types supported by the CLC Genomics Workbench, such as tracks, heat maps and PCA plots. The analysis and viewing functionalities of the CLC Sequence Viewer are also available by running the CLC Genomics Workbench, version 11.0 and higher, in Viewing Mode. It can also be used to view some of the analysis outputs of CLC commercial workbenches, with the exceptions of some more advanced data types, such as track-based data, and data stored on a CLC Genomics Server. Candidate Division SR1 and GracilibacteriaĬLC Sequence Viewer is a free, user friendly application offering access to basic bioinformatics analyses. Echinoderm Mitochondrial Flatworm Mitochondrial Ciliate Nuclear Dasycladacean Nuclear Hexamita Nuclear Mold Mitochondrial Protozoan Mitochondrial Coelenterate Mitochondrial Mycoplasma Spiroplasma ![]() Restriction enzymes database configuration.Bioinformatics explained: Multiple alignments.Bioinformatics explained: Protein statistics.Tools for linking sequence and structure.Mark molecule as circular and specify starting point.Using split views to see details of the circular molecule.Export of folders and multiple elements in CLC format.Selecting which part of the view to print.When the program is installed: Getting started.Installation on Linux with an installer.
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